In an article revealed within the journal Vitamins, authors at Florida A&M College summarized the newest analysis on the anticancer potential of kaempferol (3,4′,5,7-tetrahydroxyflavone) towards triple-negative breast most cancers (TNBC).
Sources and pharmacological results of kaempferol. Evaluation: The Anticancer Results and Therapeutic Potential of Kaempferol in Triple-Detrimental Breast Most cancers
Background
Breast most cancers is a extremely heterogeneous illness with phenotypically and genetically various cell teams. The commonest threat elements for the event of this illness embrace gender, ethnicity, genetic make-up, way of life, geographical elements, and radiation publicity.
Breast most cancers is the second main explanation for cancer-related loss of life amongst ladies in the US. About 13% of US ladies are prone to develop breast most cancers of their lifetime.
Amongst numerous subtypes, triple-negative breast most cancers (TNBC) is an aggressive phenotype accounting for about 10 – 15% of all breast most cancers instances. The absence of estrogen, progesterone, and human epidermal development receptors in TNBC makes it difficult to deal with with standardized hormone remedy.
Kaempferol metabolism
Research investigating the therapeutic potential of phytochemicals have recognized a number of pure compounds with anticancer and chemopreventive properties, together with taxanes, vinblastine, vincristine, and podophyllotoxin analogs. These compounds have additionally been discovered to mitigate chemoresistance and improve the chemo-sensitivity of most cancers cells towards commonplace chemotherapeutic brokers.
Kaempferol is a pure flavonoid with antioxidant, anti-inflammatory, anticancer, chemopreventive, cardioprotective, and neuroprotective properties. It’s generally present in greens like broccoli, capers, cabbage, onion, inexperienced peas, kale, and spinach, and in berries like strawberries, gooseberries, and blackberries.
This lipophilic compound is absorbed primarily via the small gut and extensively metabolized within the liver to generate methyl or sulfate salts. The intestinal microbiota additionally metabolizes kaempferol into aglycones and a few phenolic compounds.
All metabolites of kaempferol are absorbed into the systemic circulation, distributed in numerous tissues, and excreted in feces and urine.
Kaempferol in most cancers administration
Anti-tumorigenic, anti-proliferative, and pro-apoptotic actions of kaempferol have been noticed towards numerous most cancers varieties, together with liver, lung, colorectal, pancreatic, kidney, bone, ovarian, and breast cancers.
Kaempferol, utilized in mixture with chemotherapeutic brokers, has been discovered to extend the efficacy of chemotherapy by chemo-sensitizing most cancers cells. Kaempferol has additionally been discovered to reverse the chemoresistance of normal chemotherapeutic brokers by modulating numerous intracellular signaling pathways.
Anticancer mode of motion of kaempferol in breast most cancers
Lifelong publicity to endogenous and exogenous estrogen is understood to be related to the chance of breast most cancers growth. Regardless of being essentially the most extensively consumed dietary phytoestrogen, kaempferol has been discovered to exert anticancer exercise in each estrogen-dependent and estrogen-independent manners.
The impact of the flavonoid kaempferol on the event and development of breast most cancers. Inexperienced arrows point out induction and pink arrows point out inhibition.
In each estrogen-dependent and estrogen-independent breast most cancers cells, kaempferol has been discovered to extend DNA synthesis at low concentrations and inhibit DNA synthesis and cell development at excessive concentrations.
Kaempferol has been discovered to exert each estrogenic and anti-estrogenic actions relying on the focus used. Its estrogen agonistic exercise is understood to be related to inhibition of DNA synthesis and cell development.
Accessible proof signifies that kaempferol inhibits the proliferation of TNBC cells by inducing apoptosis and cell cycle arrest on the G2/M part. It may additionally inhibit the fatty acid synthetase enzyme to scale back cell development and induce apoptosis in breast most cancers cells overexpressing this enzyme.
Proof collected from 2D and 3D cell tradition fashions signifies that kaempferol induces breast most cancers cell apoptosis by modulating the ERK/MEK1/ELK1 signaling pathway.
Relating to focusing on estrogenic pathways, proof signifies that kaempferol antagonizes estrogen-mediated breast most cancers cell proliferation by upregulating the expression of cathepsin, cyclin D1, and cyclin E and downregulating the expression of Bax and p21.
Moreover, kaempferol has been discovered to stop the malignant transformation of breast most cancers cells by inhibiting cell proliferation through estrogen-dependent pathways.
Kaempferol, at high and low concentrations, has been discovered to scale back the viability of estrogen-positive and estrogen-negative breast most cancers cells, respectively. These results are related to lowered mRNA and protein expressions of estrogen-alpha and lowered expressions of progesterone receptor, insulin receptor, and cyclin D1.
Relating to antioxidant exercise, proof signifies that kaempferol inhibits the formation of neutrophil extracellular traps, decreasing the manufacturing of reactive oxygen species (ROS) and inhibiting breast most cancers metastasis.
Kaempferol has been discovered to synergize the anti-oxidative exercise of fisetin (one other anticancer agent) and activate gamma-H2A histone member of the family X (H2AX), resulting in DNA injury and apoptosis.
Doxorubicin, a well-established chemotherapeutic agent, is understood to induce numerous adversities, together with vascular toxicity and cardiotoxicity. Kaempferol has been discovered to reverse these adversities by regulating ROS ranges.
Relating to anti-metastatic and anti-invasive actions, proof signifies that kaempferol inhibits the activation of RhoA, Rac, and GTP-binding proteins concerned in microfilament association, resulting in inhibition of TNBC cell migration.
Kaempferol has additionally been discovered to inhibit TNBC cell adhesion, motility, and migration by decreasing the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9.
Pharmaceutical formulations of kaempferol
A number of formulations and supply programs have been developed to beat the challenges related to poor water solubility and restricted bioavailability of kaempferol.
Kaempferol-loaded nanoemulsion MNE has been developed with chitosan for muco-adhesion to realize nasal supply. The product has proven excessive permeability throughout the nasal mucosa and excessive anticancer exercise towards glioma cells.
Kaempferol gold nanoparticles have additionally proven strong biocompatibility and elevated pro-apoptotic and anti-angiogenic actions in breast most cancers cells.
Scientific proof
A scientific trial involving wholesome adults has proven {that a} every day dose of high-dose kaempferol for 4 weeks isn’t related to opposed occasions.
One other trial involving male people who smoke has proven that high-level dietary consumption of kaempferol is related to a discount in pro-inflammatory mediators.
Extra scientific trials are wanted to grasp the potential of kaempferol in most cancers administration conclusively.
